精品国产乱码一区,久草婷婷成人,大鸡巴日嫩逼视频免费网址,丝袜在线视频观看91

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2149SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓

SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓

簡(jiǎn)要描述:CRL-2149 SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2149
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-26
  • 訪  問(wèn)  量:2375

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2149 SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓

ATCC® Number:CRL-2149™  Price:$338.00
Desisgnations:SK-N-DZDepositor:C HelsonBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: brainDisease: neuroblastomaDerived from metastatic site: bone marrowCell Type: neuroblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-2149 SK-N-DZ 人成神經(jīng)瘤細(xì)胞-骨髓Tumorigenic:YesDNA Profile (STR):Amelogenin: XCSF1PO: 12D13S317: 8,11D16S539: 9,11D5S818: 12D7S820: 12,13THO1: 6,9TPOX: 8vWA: 16,18Cytogenetic Analysis:modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seenAge:2 yearsGender:femaleEthnicity:CaucasianComments:SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.Retinoic acid induces differentiation in this line.Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins.The cells exhibit moderate MDR1 expression.Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%Temperature: 37.0°CSubculturing:Protocol: Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:4 is recommendedMedium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002recommended serum:ATCC 30-2020References:22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 661079222439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 841782423127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694Related Links NCBI Entrez SearchMake a DepositFrequently Asked QuestionsMaterial Transfer AgreementTechnical SupportRelated Cell Culture Products

ATCC® Number: CRL-2149™ Price: $338.00

Designations: SK-N-DZ

Depositors: C Helson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow

Cell Type: neuroblast;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 8,11

D16S539: 9,11

D5S818: 12

D7S820: 12,13

THO1: 6,9

TPOX: 8

vWA: 16,18

Cytogenetic Analysis: modal number = 44, XX; most cells were monosomic for chromosomes 10, 11, 13, 14 and 19; all cells were missing both copies of chromosome 2; five marker chromosomes of unknown origin were observed in each cell. One of the marker chromosomes contains a homogeneously staining region (HSR); no double minutes were seen

Age: 2 years

Gender: female

Ethnicity: Caucasian

Comments: SK-N-DZ is a neuroblastoma cell line derived in 1978 from a bone marrow metastasis from a child with poorly differentiated embryonal neuroblastoma.

Retinoic acid induces differentiation in this line.

Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells.

Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins.

The cells exhibit moderate MDR1 expression.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

23127: Matsumoto M, et al. Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ. Acta Neuropathol. 79: 217-221, 1989. PubMed: 2480694

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品香港三级国产AV| 高清一区二区三区| 日本高清不卡中文字幕免费| 一本高清热品品| 国产传媒国色天香| 黄a网站| 亚洲男人的天堂在线播放| 伊人久久大香线蕉精品| 国产成人AV乱码在线观看| 日韩精品久久久免费观看| 野花社区在线视频观看| 吉水县| 玛沁县| 方正县| 广平县| 江山市| 无码一区二区波多野播放搜索 | 国产成年女人毛片80s网站| 国产精品丝袜黑色高跟鞋无码 | 亚洲熟妇av一区二区三区下载| 欧美人与动牲猛交a欧美精品| 丁香五月亚洲综合深深爱| 齐齐哈尔市| 白嫩无码人妻丰满熟妇啪啪区| 汾西县| 国产sM重味一区二区三区| 久久青青草原亚洲av无码app| 和田市| 永德县| 国产一卡2卡三卡4卡免费网站| 闵行区| 久久无码AV中文出轨人妻| 欧美xxxx做受视频| 亚洲第一av片精品堂在线观看 | 不卡高清AV手机在线观看| 最近中文字幕免费MV在线视频| 日韩免费人妻AV无码专区蜜桃| 国内精品人妻久久毛片APP| 丁香五月天综合缴情网| 国产日韩欧美亚欧在线| a级毛片成人网站免费看|