精品国产乱码一区,久草婷婷成人,大鸡巴日嫩逼视频免费网址,丝袜在线视频观看91

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-37人結(jié)腸腺癌細(xì)胞

人結(jié)腸腺癌細(xì)胞

簡(jiǎn)要描述:HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-37
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-25
  • 訪  問(wèn)  量:2226

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞

ATCC® Number: HTB-37™

Designations: Caco-2

Depositors: J Fogh

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

HTB-37 Caco-2 人結(jié)腸腺癌細(xì)胞

Source: Organ: colon

Disease: colorectal adenocarcinoma

Cellular Products: keratin

retinoic acid binding protein 1

retinol binding protein 2

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Receptors: heat stable enterotoxin (Sta, E. coli), expressed

epidermal growth factor (EGF), expressed

Virus Susceptibility: Human immunodeficiency virus 1

Tumorigenic: Yes

Reverse Transcript: N

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 11,13,14

D16S539: 12,13

D5S818: 12,13

D7S820: 11,12

TH01: 6

TPOX: 9,11

vWA: 16,18

Cytogenetic Analysis: The stemline modal chromosome number is 96, occurring at 16% with polyploidy at 3.2%. Ten common markers were detected i.e., t(1q;?), 10q-, t(11q17q) and 7 others. The t(1q17q) and M11 were found in a portion of cells. The ins(2), 10q-, and t(15q;?) were generally paired, and t(11q;17q) and t(21q;?) were mostly three-copied. Normal N9 was absent, and N21 was lost in some cells. One to 4 small acrocentric chromosomes were detected. No Y chromosome with bright distal q-band was detected by Q-observation.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 1

PGM3, 1

Age: 72 years adult

Gender: male

Ethnicity: Caucasian

HeLa Markers: N

Comments: Upon reaching confluence, the cells express characteristics of enterocytic differentiation [PubMed ID: 1939345]. Caco-2 cells express retinoic acid binding protein I and retinol binding protein II [PubMed ID: 9040537].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.The recommended inoculum is 1 X 10(4) viable cells/cm2. Subculture cells when they are about 80% confluent, at a cell concentration between 8 X 10(4) and 1 X 10(5) cell/cm2.

Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: 1 to 2 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Doubling Time: about 62 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derivative:ATCC CRL-2102

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708

22409: Jumarie C, Malo C. Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J. Cell. Physiol. 149: 24-33, 1991. PubMed: 1939345

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22564: Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

23009: Cohen MB, et al. Receptors for Escherichia coli heat stable enterotoxin in human intestine and in a human intestinal cell line (Caco-2). J. Cell. Physiol. 156: 138-144, 1993. PubMed: 8100232

HTB-37 Caco-2



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
人妻无码久久久久久久久久久| 日产乱码卡一卡2卡三卡四| 精品亚洲一区二区三区在线播放| 日本一区二区三区在线播放| 调教美丽的白丝袜麻麻| 不卡av一区二区中文字幕| 日本精品啪啪一区二区三区| 亚洲区激情区无码区日韩区| 国产无遮挡又黄又爽又色学生| 亚洲精品高潮呻吟久久av| 国产精品一区二区av| 无码精品一区二区三区四区| 国产乱人激情h在线观看| 成人做受视频试看120秒| 国内精品久久久久久蜜芽| 国产欧美另类精品久久久| 久久久久亚洲AV片无码| 色妞www精品视频| 日本日本乱码伦视频在线观看 | 欧洲无人区卡一卡二卡三| 芜湖市| 性色av一区二区| 99热热热| 一本一道久久a久久精品综合| 精品欧美黑人一区二区三区| 啪啪导航| 亚洲中文久久精品无码99| 久久av免费这里有精品| 曰韩少妇内射免费播放| 亚洲人成绝费网站色WWW| 初尝黑人巨砲波多野结衣| 亚洲欭美日韩颜射在线二| 在线观看肉片AV网站免费| 婷婷五月六月激情综合色中文字幕| 伊人久久综合无码成人网| 欧美成人精品第一区| 伊伊人成亚洲综合人网7777| 久久久久AV无码免费网| 亚洲产国偷v产偷v自拍色戒| 成年午夜精品久久久精品 | 天天摸日日摸狠狠添|